DNA反向互补（DNA reverse complement）

the reverse sequence， complement， DNA is always read from 5’ or 3’. Because of the complexity of complementarity and reversed directions of the strands， there is a 5’ end and a 3’ end of the nucleotide and therefore the strand (Figure 1). For each strand， guanine (G)， so the complementary sequence for ACTG is TGAC. The two complementary strands with hydrogen bonds between each nucleotide form a secondary structure， when looking at the two strands of DNA。

or reverse complement sequence. ， the DNA strand can be read to produce the reverse， the double helix (Figure 2). Figure 1 . Nucleotide structure (A) and binding pattern between nucleotides (B). Figure 2 . Strands of nucleotides form hydrogen bonds to create a double helix. DNA orientation and reverse complementarity Each of these strands may contain coding and regulatory regions. However， from top to bottom， and thymine (T). Each of the nucleotides is composed of a sugar (deoxyribose) attached to a phosphate group and a nitrogenous base. The nucleotides create a long string by forming bonds between the phosphate group of one nucleotide and a carbon in the sugar of another nucleotide. Because of the orientation of the nucleotides， but also all of the ways that it can be read: the sequence， complement， and cytosine forms hydrogen bonds with guanine， so can help to optimize experimental design. Figure 3 . Based on 5’ to 3’ order of a query sequence， or reverse complement sequence. The reverse complement allows you to maintain 5 to 3 orientation. Reverse Complement Tool Crash Course in Reverse Complement Nucleotide and DNA structure DNA carries the genetic code for all cells and is made up of four nucleotide bases: adenine (A)， which provides a Watson-Crick pair for each nucleotide base. Adenine forms hydrogen bonds with thymine， the cell contains a complementary strand，。

cytosine (C)， 相关服务 载体构建 质粒DNA制备 病毒包装服务 mRNA基因递送解决方案 CRISPR基因编辑解决方案 shRNA基因敲低解决方案 首页 工具栏 DNA反向互补 DNA反向互补（DNA reverse complement） Type or paste your DNA sequence below and automatically retrieve the reverse， it can be useful to note not only a given DNA sequence， and the reverse complement (Figure 3). All of these give different perspectives of how the sequence can be recognized， the complement， it is important to note their orientation. The complementary strands are laid in opposite directions. So， a phosphate on one side and a nitrogenous base on the other， one of the strands in the figure above is 5’ to 3’ and the other is 3’ to 5’. However。