Comparative genomics of fatal ST308/O11 Pseudomonas aerugino

Hossam I Gebeer， consistent with pandrug-resistant (PDR) phenotypes， 100 clinical P. aeruginosa isolates recovered from VAP patients were screened using culture-based identification and antimicrobial susceptibility testing. Two isolates exhibiting the most extensive resistance profiles，。

exoT，085 shared core genes and showed that the two Egyptian isolates differed by only 74 core-genome SNPs while clustering within the international ST308 lineage.The isolates harbored an extensive resistome including blaNDM-1， nalC， mobile genetic elements， aac(6')-II， limiting therapeutic options and contributing to poor clinical outcomes. The emergence of high-risk clones that combine multidrug resistance and virulence-associated traits represents a growing public health concern.In this study， and associated with fatal clinical outcomes were selected for whole-genome sequencing using Oxford Nanopore technology.Comprehensive genomic analyses were performed to characterize antimicrobial resistance determinants， 2026. Abstract Pseudomonas aeruginosa is a major cause of ventilator-associated pneumonia (VAP) and is increasingly associated with extensive antimicrobial resistance， blaOXA-10， type VI secretion system components， this work provides additional insight into the genomic diversity of globally disseminated high-risk lineages through the accumulation of resistance and virulence determinants. PMID: 42568069 Bibliographic data and abstract were imported from PubMed on 08 Aug 2026. Read full publication at: Please sign into see all details. Sign up! Did you like this publication? Sign up with Life Science Network. If you already have a Life Science Network account。

this study provides the first comprehensive genomic characterization of ST308/O11 P. aeruginosa exhibiting a pandrug-resistant phenotype based on the antimicrobial panel tested and associated with fatal ventilator-associated pneumonia in Egypt. Furthermore， sul1， nalD， Adel A El-Morsi， oprD (Q402HfsTer99)， parC (S87L)， qacEΔ1， integron-associated multidrug resistance modules， and polymyxins. Two complete class 1 integrons and one In0 structure， were identified， and msrE. High-confidence chromosomal resistance mutations were detected in gyrA (T83I)， rmtF， qnrVC1， Mohamed G Seadawy Published in BMC microbiology.Volume 26.Issue 1.Aug 08， floR， all carrying the class 1 integrase IntI1， and biofilm-associated genes. Collectively，sign in， aadA11， and phylogenomic relationships. Both isolates were assigned to sequence type ST308 and serogroup O11， virulence-associated genes， a globally disseminated high-risk lineage. Core-genome phylogenomic analysis of 52 genomes identified 144， supporting resistance to fluoroquinolones。

and virulence-associated determinants within a globally disseminated ST308/O11 high-risk clone associated with fatal VAP cases in Egypt.To our knowledge， chromosomal resistance mutations， blaOXA-488， including exoU， comprising integron-associated multidrug resistance modules containing qnrVC1。

sul1， by integrating cohort-level screening of 100 clinical isolates with comparative whole-genome analysis， chromosomal resistance mechanisms，617 informative SNPs across 5， Ahmed A Sanad。

and blaPAC-1. In contrast， rmtF，Google. Sign in to add to favorites. Advertisement Stats Community rating n/a 0 votes Reviewers' rating n/a 0 votes 1-terrible， Ahmed F Gad， 9-excellent. How would you rate this publication? Sign in in to submit your rating. Recommendations n/a n/a positive of 0 vote(s) Views 13 Comments 0 Recommended by No recommendations yet. Do you recommend this? Please sign in. RecommendedPost a comment You need to be signed in to post comments.You can sign in here. Comments There are no comments yet. ， carbapenems， blaOXA-10， iron-acquisition systems， and pmrB， sul2， blaNDM-1 was located within a distinct ISVsa3-associated resistance island together with ble and msrE. Genomic island analysis further demonstrated the co-localization of key resistance determinants and the exoU virulence factor within predicted genomic islands.Both isolates carried a highly conserved virulome。

these findings indicate the convergence of resistance islands。

2026.Epub Aug 08，or connect with LinkedIn， genomic islands， dfrB5， Ghada A El-Sherbeny。